Optimization of a digestion method to determine total mercury in fish tissue by cold vapor atomic fluorescence spectrophotometry

Gabriela S. Yánez-Jácome, David Romero-Estévez, Hugo Navarrete, Karina Simbaña-Farinango, Pamela Y. Vélez-Terreros

Producción científica: Contribución a una revistaArtículorevisión exhaustiva

8 Citas (Scopus)

Resumen

Several microwave-assisted digestion methods were tested at the Centro de Estudios Aplicados en Química laboratory in Quito, Ecuador, to determine the accuracy and performance efficiency of the mineralization process for the determination of total mercury in fish tissue by cold vapor atomic fluorescence spectrophotometry. The use of MARSEasyPrep high-pressure vessels, low amounts of reagents (1 cm3 HNO3, 1 cm3 H2O2, and 1 cm3 HClO4), an irradiation temperature of 210C, and 35 min of mineralization time resulted in accurate performance, with recoveries of certified reference material DORM-4 between 90.1% and 105.8%. This is better than the Association of Official Analytical Chemists 2015.01 method, which has a reported accuracy of 81%. The repeatability precision and intermediate precision were established at three concentration levels (0.167, 0.500, and 0.833 mg·kg−1) and expressed as the percentage of the relative standard deviation ranging from 1.5% to 3.0% and 1.7% to 4.2%, respectively. Further, the method was satisfactorily applied to analyze fortified samples of tilapia (Oreochromis niloticus), with recoveries ranging from 98.3% to 104.3%. The instrumental limits of detection and quantification were 0.118 µg·dm−3 and 0.394 µg·dm−3, respectively.

Idioma originalInglés
Número de artículo45
Páginas (desde-hasta)1-12
Número de páginas12
PublicaciónMethods and Protocols
Volumen3
N.º2
DOI
EstadoPublicada - 23 jun. 2020

Nota bibliográfica

Publisher Copyright:
© 2020 by the authors. Licensee MDPI, Basel, Switzerland.

Huella

Profundice en los temas de investigación de 'Optimization of a digestion method to determine total mercury in fish tissue by cold vapor atomic fluorescence spectrophotometry'. En conjunto forman una huella única.

Citar esto