METHOD FOR OBTAINING SULFATED NANOPARTICLES OF AND MANNAN FROM ENDOSPERMS

A method for obtaining highly sulfated nanoparticles of cellulose or sulfated nanoparticles of mannan, or a combination thereof, from endosperms, comprising acquiring a ground flour of an endosperm of a seed, nut, or pit, wherein the ground flour contains polysaccharides, and wherein the polysaccharides contain either cellulose or mannan, or both cellulose and mannan; pretreating the ground flour to condition the polysaccharides for hydrolysis and functionalization thereof; preparing a reagent for hydrolysis and functionalization of the polysaccharides, wherein the reagent comprises a solution of glyeerol, water, and sulfuric acid at predetermined concentrations; adding the reagent to the pretreated polysaccharides to form a suspension, wherein adding the reagent to the suspension convens the cellulose
or mannan or both into nanoparticles that are sulfatexl by the reagent; separating the sulfated nanoparticles fmrn the suspension; washing the nanoparlicles; and creating a stable colloidal suspension of the sulfáted nanoparticles. 'Ill•e sulfated nanoparticles are used in pharmaeeutical, cosmetic, wound healing, and skin treatment formulations.