Immunological characterization of antigens released by Trypanosoma cruzi-infected cells

Mario J. Grijalva, Kenneth J. Goodrum, Edwin C. Rowland

Producción científica: Contribución a una revistaArtículorevisión exhaustiva

8 Citas (Scopus)


Chagas' disease, caused by Trypanosoma cruzi, is characterized by the appearance of pathological lesions in the heart and other tissues during the chronic phase. The mechanisms responsible for such damage are still unclear. In the vertebrate host, T. cruzi replicates intracellularly before transforming from amastigotes into trypomastigotes. The infected host cell then lyses, releasing the cytoplasmic contents and the parasites that shed membrane glycoproteins soon after release. The sum of all these components we have termed released antigen (Rag). We characterized antigens, released in vitro by fibroblasts infected with T. cruzi, obtained by concentrating conditioned serum-free culture media. The results demonstrate that Rag contains a complex protein mixture including stage-specific T. cruzi antigens (Ssp-1, -2, -4), glucose-regulated protein (Grp) 78h, and peptides recognized by the monoclonal antibody 2B10. These peptides exhibit neuraminidase activity and are expressed by intracellular and 10~20% of released trypomastigotes. Additionally, Rag is recognized by sera from T. cruzi- infected mice and human chagasic patients. Rag also stimulates in vitro production of interferon-γ by splenocytes from resistant C57B1/6 and susceptible BALB/c infected mice and interleukin-4 by splenocytes from BALB/c infected mice. Altogether these results indicate that Rag is immunologically relevant and could contribute to pathogenesis of T. cruzi infection.

Idioma originalInglés
Páginas (desde-hasta)663-671
Número de páginas9
PublicaciónJournal of Parasitology
EstadoPublicada - ago. 1999
Publicado de forma externa


Profundice en los temas de investigación de 'Immunological characterization of antigens released by Trypanosoma cruzi-infected cells'. En conjunto forman una huella única.

Citar esto