TY - JOUR
T1 - Development of gastro-resistant coated probiotic granulates and evaluation of viability and release during simulated upper gastrointestinal transit
AU - Jacobsen, Natashia Mai Yde
AU - Nedergaard, Hanne Bjørn
AU - Kock, Anette
AU - Caglayan, Ibrahim
AU - Laursen, Marie Munch
AU - Lange, Eva Marie
AU - Marcial-Coba, Martín Sebastián
AU - Bar-Shalom, Daniel
AU - Nielsen, Dennis Sandris
AU - Müllertz, Anette
N1 - Publisher Copyright:
© 2021
PY - 2021/6
Y1 - 2021/6
N2 - Probiotics have become one of the most consumed food supplements worldwide. Many probiotic strains are sensitive to low pH and bile concentrations encountered in the gastrointestinal tract upon oral ingestion. This study aimed at developing gastro-resistant probiotic granulates releasing viable cells in the small intestine. Fatty alcohols were investigated as potential coating materials. Cetostearyl alcohol was selected and combined with different plasticizers to further optimize the coating properties. Combining cetostearyl alcohol with olive oil and beeswax in selected concentrations was found promising, and the coatings were applied to L. acidophilus LA3 and B. longum BB536, using hot-melt fluid bed coating. Viability in and release from the coated probiotic granulate was investigated, using a physiological relevant in vitro model simulating conditions in the human stomach, duodenum, jejunum and ileum. L. acidophilus LA3 coated with cetostearyl alcohol and olive oil in the ratio 95:5 (w/w) resulted in significantly higher viability after simulated gastrointestinal transit, compared to the uncoated probiotic powder. Furthermore, the coating showed no release of viable cells after simulated gastric transit. Released viable cells were detected after the remaining steps in the in vitro model, indicating that the coating system provides gastric protection and release during intestinal transit.
AB - Probiotics have become one of the most consumed food supplements worldwide. Many probiotic strains are sensitive to low pH and bile concentrations encountered in the gastrointestinal tract upon oral ingestion. This study aimed at developing gastro-resistant probiotic granulates releasing viable cells in the small intestine. Fatty alcohols were investigated as potential coating materials. Cetostearyl alcohol was selected and combined with different plasticizers to further optimize the coating properties. Combining cetostearyl alcohol with olive oil and beeswax in selected concentrations was found promising, and the coatings were applied to L. acidophilus LA3 and B. longum BB536, using hot-melt fluid bed coating. Viability in and release from the coated probiotic granulate was investigated, using a physiological relevant in vitro model simulating conditions in the human stomach, duodenum, jejunum and ileum. L. acidophilus LA3 coated with cetostearyl alcohol and olive oil in the ratio 95:5 (w/w) resulted in significantly higher viability after simulated gastrointestinal transit, compared to the uncoated probiotic powder. Furthermore, the coating showed no release of viable cells after simulated gastric transit. Released viable cells were detected after the remaining steps in the in vitro model, indicating that the coating system provides gastric protection and release during intestinal transit.
KW - Fluid bed
KW - Hot-melt coating
KW - In vitro simulation
KW - Probiotic delivery systems
KW - Probiotics
UR - http://www.scopus.com/inward/record.url?scp=85101987357&partnerID=8YFLogxK
U2 - 10.1016/j.lwt.2021.111174
DO - 10.1016/j.lwt.2021.111174
M3 - Article
AN - SCOPUS:85101987357
SN - 0023-6438
VL - 144
JO - LWT
JF - LWT
M1 - 111174
ER -