Determinación de la viabilidad de los concentrados plaquetarios obtenidos de capa leucocitaria (Buffy coat) durante siete días de almacenamiento

Clara E. Arroyo Rubio, Katherine E. Jaramillo Ruales, Rosa F. Chiriboga Ponce

Producción científica: Contribución a una revistaArtículorevisión exhaustiva

Resumen

Introduction: Platelet concentrates (cpq) are labile blood components affected by several factors from the method of production to storage conditions that cause a gradual loss of functionality. For this reason, it is necessary to evaluate the platelet quality parameters that guarantee the viability during the storage days, with the purpose of monitoring the maintenance of the functional characteristics of the platelets. Materials and methods: This cross-sectional descriptive study had a sample size of 64 platelet concen-trates, evaluated at 3, 5, and 7 days of storage. The monitored parameters were the physical storage parameters and percentage of platelet activation by measuring P-selectin (cd62) via flow cytometry. The chi-square statistic, one-way Anova, Kruskal–Wallis test, and Pearson correlation were applied. Results: Significant differences were observed on the 7th day in relation to the 3rd and 5th day of storage, espe-cially in the swirling parameter (p < 0.005) and platelet aggregates (p = 0.001). The platelet activation increased significantly (p = 0.001) on the 5th day. Conclusions: Based on the findings of this study, the viability of the platelet concentrates differs with the days of storage. For this reason, it is necessary to evaluate the swirling, pH, and aggregates to all platelet concentrates before being transfused as an indication of platelet activation and decreased functionality.

Título traducido de la contribuciónMonitoring of the viability of platelet concentrates obtained from buffy coat during seven days of storage
Idioma originalEspañol (Ecuador)
PublicaciónRevista Ciencias de la Salud
Volumen19
N.º3
DOI
EstadoPublicada - 20 sep. 2021

Nota bibliográfica

Publisher Copyright:
© 2021, Universidad del Rosario. All rights reserved.

Palabras clave

  • Buffy coat
  • Platelet concentrate
  • Validation
  • Viability

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