Chromosome evolution in Lophyohylini (Amphibia, Anura, Hylinae)

Pablo Suárez; Juan M. Ferro; Cleusa Y. Nagamachi; Dario E. Cardozo; Ailin Blasco-Zúñiga; Jéssica B. Silva; Euvaldo Marciano; Marco A. Costa; Victor G.D. Orrico; Mirco Solé; Igor J. Roberto; Miryan Rivera; John E. Wiley; Julián Faivovich; Diego Baldo; Julio C. Pieczarka

doi:10.1371/journal.pone.0234331

Chromosome evolution in Lophyohylini (Amphibia, Anura, Hylinae)

Pablo Suárez, Juan M. Ferro^*, Cleusa Y. Nagamachi, Dario E. Cardozo, Ailin Blasco-Zúñiga, Jéssica B. Silva, Euvaldo Marciano, Marco A. Costa, Victor G.D. Orrico, Mirco Solé, Igor J. Roberto, Miryan Rivera, John E. Wiley, Julián Faivovich, Diego Baldo^*, Julio C. Pieczarka

^*Autor correspondiente de este trabajo

Facultad de Ciencias Exactas, Naturales y Ambientales

Producción científica: Contribución a una revista › Artículo › revisión exhaustiva

5 Citas (Scopus)

Resumen

The hyline tribe Lophyohylini includes 87 species of treefrogs, of which cytogenetics aspects have been studied in less than 20% of them. In order to evaluate the evolution of some of its chromosome characters (NOR position, C-bands, and DAPI/CMA₃ bands), we studied the karyotypes of 21 lophyohylines, 16 of them for the first time, and analyzed them in a phylogenetic context. Most species showed similar karyotypes regarding chromosome number (2n = 24) and morphology (FN = 48), excepting Phyllodytes edelmoi and Osteocephalus buckleyi with 2n = 22 (FN = 44) and 2n = 28 (FN = 50), respectively. The NOR location was variable among species and provided valuable phylogenetic information. This marker was located in pair 11 in all species of Trachycephalus, Itapotihyla langsdorffii, and Nyctimantis arapapa, representing the plesiomorphic condition of Lophyohylini. Besides, other apomorphic states were recovered for the clades comprising N. rugiceps and N. siemersi (NOR in pair 5), and Dryaderces pearsoni, Osteocephalus, and Osteopilus (NOR in pair 9). Phyllodytes presented variation for NORs position; they were in pair 2 in P. edelmoi, pair 7 in P. melanomystax, and pair 8 in P. gyrinaethes and P. praeceptor. Polymorphisms in size, number, and activity of this marker were observed for N. siemersi, Osteocephalus fuscifacies, and some species of Trachycephalus. Remarkably, in N. siemersi NORs were detected on a single chromosome in the two specimens studied by this technique, raising the question of how this complex polymorphism is maintained. Interstitial telomeric sequences were found in P. edelmoi,P. melanomystax, and Osteocephalus buckleyi, and their presence seems to be not related to the chromosome reorganization events. Finally, some species showed spontaneous rearrangements, possibly as a consequence of an uncommon phenomenon in anuran cytogenetics: the presence of fragile sites or secondary constrictions not associated with NORs. We propose that this rare feature would have played an important role in the evolution of this group of frogs. From the evidence obtained in this and previous studies, we conclude that Lophyohylini presents a complex chromosome evolution.

Idioma original	Inglés
Número de artículo	e0234331
Publicación	PLoS ONE
Volumen	15
N.º	6 1
DOI	https://doi.org/10.1371/journal.pone.0234331
Estado	Publicada - 11 jun. 2020

Nota bibliográfica

Publisher Copyright:
© 2020 Suárez et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Financiación

Financiadores	Número del financiador
John Merck Fund
Willi Hennig Society
Coordenação de Aperfeiçoamento de Pessoal de Nível Superior	110447, 047/ 2012, 552032/2010-7, ICAAF 007/2011
Consejo Nacional de Investigaciones Científicas y Técnicas
Agencia Nacional de Promoción Científica y Tecnológica	PICTs 2013-404, 2015-820, 2015-2381, PICT 2018-3349
Conselho Nacional de Desenvolvimento Científico e Tecnológico
Fundação Amazônia Paraense de Amparo à Pesquisa
Fundação de Amparo à Pesquisa do Estado da Bahia	8952/2014
Fundação de Amparo à Pesquisa e Inovação do Espírito Santo	78252482, 310467/2017-9, 88882.156872/2016-01
Universidade Federal do Pará
Pontificia Universidad Católica del Ecuador	QINV0046 - IINV529010100
Universidad Nacional de Misiones	16Q001-TI
Banco Nacional de Desenvolvimento Econômico e Social	2.318.697.0001

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10.1371/journal.pone.0234331

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Suárez, P., Ferro, J. M., Nagamachi, C. Y., Cardozo, D. E., Blasco-Zúñiga, A., Silva, J. B., Marciano, E., Costa, M. A., Orrico, V. G. D., Solé, M., Roberto, I. J., Rivera, M., Wiley, J. E., Faivovich, J., Baldo, D., & Pieczarka, J. C. (2020). Chromosome evolution in Lophyohylini (Amphibia, Anura, Hylinae). PLoS ONE, 15(6 1), Artículo e0234331. https://doi.org/10.1371/journal.pone.0234331

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title = "Chromosome evolution in Lophyohylini (Amphibia, Anura, Hylinae)",

abstract = "The hyline tribe Lophyohylini includes 87 species of treefrogs, of which cytogenetics aspects have been studied in less than 20% of them. In order to evaluate the evolution of some of its chromosome characters (NOR position, C-bands, and DAPI/CMA3 bands), we studied the karyotypes of 21 lophyohylines, 16 of them for the first time, and analyzed them in a phylogenetic context. Most species showed similar karyotypes regarding chromosome number (2n = 24) and morphology (FN = 48), excepting Phyllodytes edelmoi and Osteocephalus buckleyi with 2n = 22 (FN = 44) and 2n = 28 (FN = 50), respectively. The NOR location was variable among species and provided valuable phylogenetic information. This marker was located in pair 11 in all species of Trachycephalus, Itapotihyla langsdorffii, and Nyctimantis arapapa, representing the plesiomorphic condition of Lophyohylini. Besides, other apomorphic states were recovered for the clades comprising N. rugiceps and N. siemersi (NOR in pair 5), and Dryaderces pearsoni, Osteocephalus, and Osteopilus (NOR in pair 9). Phyllodytes presented variation for NORs position; they were in pair 2 in P. edelmoi, pair 7 in P. melanomystax, and pair 8 in P. gyrinaethes and P. praeceptor. Polymorphisms in size, number, and activity of this marker were observed for N. siemersi, Osteocephalus fuscifacies, and some species of Trachycephalus. Remarkably, in N. siemersi NORs were detected on a single chromosome in the two specimens studied by this technique, raising the question of how this complex polymorphism is maintained. Interstitial telomeric sequences were found in P. edelmoi,P. melanomystax, and Osteocephalus buckleyi, and their presence seems to be not related to the chromosome reorganization events. Finally, some species showed spontaneous rearrangements, possibly as a consequence of an uncommon phenomenon in anuran cytogenetics: the presence of fragile sites or secondary constrictions not associated with NORs. We propose that this rare feature would have played an important role in the evolution of this group of frogs. From the evidence obtained in this and previous studies, we conclude that Lophyohylini presents a complex chromosome evolution.",

author = "Pablo Su{\'a}rez and Ferro, {Juan M.} and Nagamachi, {Cleusa Y.} and Cardozo, {Dario E.} and Ailin Blasco-Z{\'u}{\~n}iga and Silva, {J{\'e}ssica B.} and Euvaldo Marciano and Costa, {Marco A.} and Orrico, {Victor G.D.} and Mirco Sol{\'e} and Roberto, {Igor J.} and Miryan Rivera and Wiley, {John E.} and Juli{\'a}n Faivovich and Diego Baldo and Pieczarka, {Julio C.}",

note = "Publisher Copyright: {\textcopyright} 2020 Su{\'a}rez et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.",

year = "2020",

month = jun,

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doi = "10.1371/journal.pone.0234331",

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T1 - Chromosome evolution in Lophyohylini (Amphibia, Anura, Hylinae)

AU - Suárez, Pablo

AU - Ferro, Juan M.

AU - Nagamachi, Cleusa Y.

AU - Cardozo, Dario E.

AU - Blasco-Zúñiga, Ailin

AU - Silva, Jéssica B.

AU - Marciano, Euvaldo

AU - Costa, Marco A.

AU - Orrico, Victor G.D.

AU - Solé, Mirco

AU - Roberto, Igor J.

AU - Rivera, Miryan

AU - Wiley, John E.

AU - Faivovich, Julián

AU - Baldo, Diego

AU - Pieczarka, Julio C.

N1 - Publisher Copyright: © 2020 Suárez et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

PY - 2020/6/11

Y1 - 2020/6/11

N2 - The hyline tribe Lophyohylini includes 87 species of treefrogs, of which cytogenetics aspects have been studied in less than 20% of them. In order to evaluate the evolution of some of its chromosome characters (NOR position, C-bands, and DAPI/CMA3 bands), we studied the karyotypes of 21 lophyohylines, 16 of them for the first time, and analyzed them in a phylogenetic context. Most species showed similar karyotypes regarding chromosome number (2n = 24) and morphology (FN = 48), excepting Phyllodytes edelmoi and Osteocephalus buckleyi with 2n = 22 (FN = 44) and 2n = 28 (FN = 50), respectively. The NOR location was variable among species and provided valuable phylogenetic information. This marker was located in pair 11 in all species of Trachycephalus, Itapotihyla langsdorffii, and Nyctimantis arapapa, representing the plesiomorphic condition of Lophyohylini. Besides, other apomorphic states were recovered for the clades comprising N. rugiceps and N. siemersi (NOR in pair 5), and Dryaderces pearsoni, Osteocephalus, and Osteopilus (NOR in pair 9). Phyllodytes presented variation for NORs position; they were in pair 2 in P. edelmoi, pair 7 in P. melanomystax, and pair 8 in P. gyrinaethes and P. praeceptor. Polymorphisms in size, number, and activity of this marker were observed for N. siemersi, Osteocephalus fuscifacies, and some species of Trachycephalus. Remarkably, in N. siemersi NORs were detected on a single chromosome in the two specimens studied by this technique, raising the question of how this complex polymorphism is maintained. Interstitial telomeric sequences were found in P. edelmoi,P. melanomystax, and Osteocephalus buckleyi, and their presence seems to be not related to the chromosome reorganization events. Finally, some species showed spontaneous rearrangements, possibly as a consequence of an uncommon phenomenon in anuran cytogenetics: the presence of fragile sites or secondary constrictions not associated with NORs. We propose that this rare feature would have played an important role in the evolution of this group of frogs. From the evidence obtained in this and previous studies, we conclude that Lophyohylini presents a complex chromosome evolution.

AB - The hyline tribe Lophyohylini includes 87 species of treefrogs, of which cytogenetics aspects have been studied in less than 20% of them. In order to evaluate the evolution of some of its chromosome characters (NOR position, C-bands, and DAPI/CMA3 bands), we studied the karyotypes of 21 lophyohylines, 16 of them for the first time, and analyzed them in a phylogenetic context. Most species showed similar karyotypes regarding chromosome number (2n = 24) and morphology (FN = 48), excepting Phyllodytes edelmoi and Osteocephalus buckleyi with 2n = 22 (FN = 44) and 2n = 28 (FN = 50), respectively. The NOR location was variable among species and provided valuable phylogenetic information. This marker was located in pair 11 in all species of Trachycephalus, Itapotihyla langsdorffii, and Nyctimantis arapapa, representing the plesiomorphic condition of Lophyohylini. Besides, other apomorphic states were recovered for the clades comprising N. rugiceps and N. siemersi (NOR in pair 5), and Dryaderces pearsoni, Osteocephalus, and Osteopilus (NOR in pair 9). Phyllodytes presented variation for NORs position; they were in pair 2 in P. edelmoi, pair 7 in P. melanomystax, and pair 8 in P. gyrinaethes and P. praeceptor. Polymorphisms in size, number, and activity of this marker were observed for N. siemersi, Osteocephalus fuscifacies, and some species of Trachycephalus. Remarkably, in N. siemersi NORs were detected on a single chromosome in the two specimens studied by this technique, raising the question of how this complex polymorphism is maintained. Interstitial telomeric sequences were found in P. edelmoi,P. melanomystax, and Osteocephalus buckleyi, and their presence seems to be not related to the chromosome reorganization events. Finally, some species showed spontaneous rearrangements, possibly as a consequence of an uncommon phenomenon in anuran cytogenetics: the presence of fragile sites or secondary constrictions not associated with NORs. We propose that this rare feature would have played an important role in the evolution of this group of frogs. From the evidence obtained in this and previous studies, we conclude that Lophyohylini presents a complex chromosome evolution.

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