WOULD TROPICAL CLIMATIC VARIATIONS IMPACT THE GENETIC VARIABILITY OF TRIATOMINES: RHODNIUS ECUADORIENSIS, PRINCIPAL VECTOR OF CHAGAS DISEASE IN ECUADOR?: Rhodnius ecuadoriensis, principal vector of Chagas disease in Ecuador?

Anita G. Villacís; Juan José Bustillos; Stéphanie Depickère; Dino Sánchez; César A. Yumiseva; Ana Troya-Zuleta; Christian Barnabé; Mario J. Grijalva; Simone Frédérique Brenière

doi:10.1016/j.actatropica.2020.105530

WOULD TROPICAL CLIMATIC VARIATIONS IMPACT THE GENETIC VARIABILITY OF TRIATOMINES: RHODNIUS ECUADORIENSIS, PRINCIPAL VECTOR OF CHAGAS DISEASE IN ECUADOR? Rhodnius ecuadoriensis, principal vector of Chagas disease in Ecuador?

Anita G. Villacís^*, Juan José Bustillos, Stéphanie Depickère, Dino Sánchez, César A. Yumiseva, Ana Troya-Zuleta, Christian Barnabé, Mario J. Grijalva, Simone Frédérique Brenière

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

7 Scopus citations

Abstract

Rhodnius ecuadoriensis is one of the most important vector species of Chagas disease in Ecuador. This species is distributed in the Central coast region and in the south Andean region, and an incipient speciation process between these geographical populations was previously proposed. The current population genetics study only focused on the Central coast region and analyzed 96 sylvatic specimens of R. ecuadoriensis associated with Phytelephas aequatorialis palm trees. We used Cytb and 16S-rRNA sequences and a Cytb-16S-rRNA concatenated set to explore (i) the genetic variability, spatial structuring, and demographic history of R. ecuadoriensis, and to determine (ii) the relationship between the genetic and climatic variabilities. A particularly high genetic variability was observed without detectable general genetic structure; only some terminal genetic clusters were observed. We did not observe isolation by geographical distance (IBD), and it is likely that ancient expansion occurred, according to Fs index and mismatch distribution for Cytb-16S-rRNA concatenated sequences. Hierarchical clustering showed that the current locality origins of the bugs were grouped into four bioclimatic clusters. Genetic and bioclimatic distances were not correlated, but some genetic clusters were associated with bioclimatic ones. The results showed an ancient evolution of the species in the region with a possible old expansion. The absence of spatial genetic structure could be due to climatic conditions (possible selection of singular genotypes) and to passive transportation of palms tree materials where R. ecuadoriensis are living.

Original language	English
Article number	105530
Journal	Acta Tropica
Volume	209
DOIs	https://doi.org/10.1016/j.actatropica.2020.105530
State	Published - Sep 2020

Bibliographical note

Publisher Copyright:
© 2020

Funding

The study received Financial support from Ohio University Heritage College of Osteopathic Medicine, Pontificia Universidad Católica del Ecuador Projects (J13039, and L13254), the European Commission Framework Programme 7 Project ‘‘Comparative epidemiology of genetic lineages of Trypanosoma cruzi’’ ChagasEpiNet, Contract No. 223034 and the National Institutes of Health , Fogarty International Center , Global Infectious Disease Training Grant ( 1D43TW008261-01A1 ). AGV: participated in directed entomological collection and identified triatomine species, Also in the study design, analyzed, interpreted results, drafted the manuscript and provided overall coordination of the study. JJB: participated in directed entomological collection and identified triatomine species, performed PCRs, analyzed results and helped draft the manuscript. SD: participated in the analyzed, interpreted results, drafted the manuscript. DS: participated in directed entomological collection and identified triatomine species and helped draft the manuscript. CAY: participated in directed entomological collection, data analysis and interpretation. ATZ: participated in the study design, analyzed and interpreted results. CB: participated in the analyzed and interpreted results. MJG: conceived the study, and helped draft the manuscript. SFB: participated in study design and guided data analysis and interpretation. Also, in the draft of the manuscript. All authors read an approved the final manuscript. Special thanks to the personnel of the National Chagas Control Program from the Ministry of Health of Ecuador who participated in the collection of the triatomines in the sylvatic area. The study received Financial support from Ohio University Heritage College of Osteopathic Medicine, Pontificia Universidad Cat?lica del Ecuador Projects (J13039, and L13254), the European Commission Framework Programme 7 Project ??Comparative epidemiology of genetic lineages of Trypanosoma cruzi?? ChagasEpiNet, Contract No. 223034 and the National Institutes of Health, Fogarty International Center, Global Infectious Disease Training Grant (1D43TW008261-01A1).

Funders	Funder number
European Commission Framework Programme 7
European Commission Framework Programme 7 Project	223034
Ohio University Heritage College of Osteopathic Medicine
National Institutes of Health
Fogarty International Center	1D43TW008261-01A1
Ministeriet Sundhed Forebyggelse
Pontifical Catholic University of Ecuador	L13254, J13039

Keywords

Central coast region
Chagas disease
Climate variability
Ecuador
Gene sequencing
Genetic variability
Triatomine

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Villacís, A. G., Bustillos, J. J., Depickère, S., Sánchez, D., Yumiseva, C. A., Troya-Zuleta, A., Barnabé, C., Grijalva, M. J., & Brenière, S. F. (2020). WOULD TROPICAL CLIMATIC VARIATIONS IMPACT THE GENETIC VARIABILITY OF TRIATOMINES: RHODNIUS ECUADORIENSIS, PRINCIPAL VECTOR OF CHAGAS DISEASE IN ECUADOR? Rhodnius ecuadoriensis, principal vector of Chagas disease in Ecuador? Acta Tropica, 209, Article 105530. https://doi.org/10.1016/j.actatropica.2020.105530

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title = "WOULD TROPICAL CLIMATIC VARIATIONS IMPACT THE GENETIC VARIABILITY OF TRIATOMINES: RHODNIUS ECUADORIENSIS, PRINCIPAL VECTOR OF CHAGAS DISEASE IN ECUADOR?: Rhodnius ecuadoriensis, principal vector of Chagas disease in Ecuador?",

abstract = "Rhodnius ecuadoriensis is one of the most important vector species of Chagas disease in Ecuador. This species is distributed in the Central coast region and in the south Andean region, and an incipient speciation process between these geographical populations was previously proposed. The current population genetics study only focused on the Central coast region and analyzed 96 sylvatic specimens of R. ecuadoriensis associated with Phytelephas aequatorialis palm trees. We used Cytb and 16S-rRNA sequences and a Cytb-16S-rRNA concatenated set to explore (i) the genetic variability, spatial structuring, and demographic history of R. ecuadoriensis, and to determine (ii) the relationship between the genetic and climatic variabilities. A particularly high genetic variability was observed without detectable general genetic structure; only some terminal genetic clusters were observed. We did not observe isolation by geographical distance (IBD), and it is likely that ancient expansion occurred, according to Fs index and mismatch distribution for Cytb-16S-rRNA concatenated sequences. Hierarchical clustering showed that the current locality origins of the bugs were grouped into four bioclimatic clusters. Genetic and bioclimatic distances were not correlated, but some genetic clusters were associated with bioclimatic ones. The results showed an ancient evolution of the species in the region with a possible old expansion. The absence of spatial genetic structure could be due to climatic conditions (possible selection of singular genotypes) and to passive transportation of palms tree materials where R. ecuadoriensis are living.",

keywords = "Central coast region, Chagas disease, Climate variability, Ecuador, Gene sequencing, Genetic variability, Triatomine",

author = "Villac{\'i}s, {Anita G.} and Bustillos, {Juan Jos{\'e}} and St{\'e}phanie Depick{\`e}re and Dino S{\'a}nchez and Yumiseva, {C{\'e}sar A.} and Ana Troya-Zuleta and Christian Barnab{\'e} and Grijalva, {Mario J.} and Breni{\`e}re, {Simone Fr{\'e}d{\'e}rique}",

note = "Publisher Copyright: {\textcopyright} 2020",

year = "2020",

month = sep,

doi = "10.1016/j.actatropica.2020.105530",

language = "English",

volume = "209",

journal = "Acta Tropica",

issn = "0001-706X",

publisher = "Elsevier B.V.",

}

Villacís, AG, Bustillos, JJ, Depickère, S, Sánchez, D, Yumiseva, CA , Troya-Zuleta, A, Barnabé, C, Grijalva, MJ & Brenière, SF 2020, 'WOULD TROPICAL CLIMATIC VARIATIONS IMPACT THE GENETIC VARIABILITY OF TRIATOMINES: RHODNIUS ECUADORIENSIS, PRINCIPAL VECTOR OF CHAGAS DISEASE IN ECUADOR? Rhodnius ecuadoriensis, principal vector of Chagas disease in Ecuador?', Acta Tropica, vol. 209, 105530. https://doi.org/10.1016/j.actatropica.2020.105530

WOULD TROPICAL CLIMATIC VARIATIONS IMPACT THE GENETIC VARIABILITY OF TRIATOMINES: RHODNIUS ECUADORIENSIS, PRINCIPAL VECTOR OF CHAGAS DISEASE IN ECUADOR? Rhodnius ecuadoriensis, principal vector of Chagas disease in Ecuador? / Villacís, Anita G.; Bustillos, Juan José; Depickère, Stéphanie et al.
In: Acta Tropica, Vol. 209, 105530, 09.2020.

Research output: Contribution to journal › Article › peer-review

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AU - Villacís, Anita G.

AU - Bustillos, Juan José

AU - Depickère, Stéphanie

AU - Sánchez, Dino

AU - Yumiseva, César A.

AU - Troya-Zuleta, Ana

AU - Barnabé, Christian

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AU - Brenière, Simone Frédérique

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Villacís AG, Bustillos JJ, Depickère S, Sánchez D, Yumiseva CA , Troya-Zuleta A et al. WOULD TROPICAL CLIMATIC VARIATIONS IMPACT THE GENETIC VARIABILITY OF TRIATOMINES: RHODNIUS ECUADORIENSIS, PRINCIPAL VECTOR OF CHAGAS DISEASE IN ECUADOR? Rhodnius ecuadoriensis, principal vector of Chagas disease in Ecuador? Acta Tropica. 2020 Sep;209:105530. doi: 10.1016/j.actatropica.2020.105530