Abstract
Ecnomiohyla tuberculosa is an Amazonian hylid of uncertain phylogenetic position. Herein DNA sequences of mitochondrial and nuclear genes are used to determine its phylogenetic relationships. New sequences and external morphology of Trachycephalus typhonius are also analyzed to assess the status of Ecuadorian and Peruvian populations. The phylogeny shows unequivocally that Ecnomiohyla tuberculosa is nested within the genus Tepuihyla, tribe Lophiohylini. This position was unexpected because the remaining species of Ecnomiohyla belong to the tribe Hylini. To solve the paraphyly of the genus Ecnomiohyla, E. tuberculosa is transferred to the genus Tepuihyla. Comparisons of DNA sequences, external morphology, and advertisement calls between populations of Ecnomiohyla tuberculosa from Ecuador and Peru indicate that the Peruvian population represents an undescribed species. The new species is described and a species account is provided for Ecnomiohyla tuberculosa. Trachycephalus typhonius is paraphyletic relative to T. cunauaru, T. hadroceps, and T. resinifictrix. The phylogenetic position of populations from western Ecuador indicates that they represent a species separate from T. typhonius sensu stricto. We resurrect the name Hyla quadrangulum (Trachycephalus quadrangulum comb. n.) for those populations. Amazonian populations of "T. typhonius" from Ecuador and Peru are genetically and morphologically distinct from T. typhonius sensu stricto and are conspecific with the holotype of Hyla macrotis. Therefore, we also resurrect Hyla macrotis, a decision that results in Trachycephalus macrotis comb. n.
| Original language | English |
|---|---|
| Pages (from-to) | 115-154 |
| Number of pages | 40 |
| Journal | ZooKeys |
| Volume | 2016 |
| Issue number | 630 |
| DOIs | |
| State | Published - 2016 |
Bibliographical note
Publisher Copyright:© Santiago R. Ron et al.
Funding
Field and laboratory work in Ecuador were funded by grants from Secretaría Nacional de Educación Superior, Ciencia, Tecnología e Innovación (SENESCYT, Arca de Noé Initiative) and PUCE-DGA (SRR principal investigator). Extraction and amplification of DNA was carried out by M. Caminer, D. Flores, A. Manzano, M. J. Nichols, and M. E. Ordoñez. Daniel Baudain, Alessandro Catenazzi, A. del Campo, Marcelo Gordo, Antoine Fouquet, and Vincent Premel provided photographs of Tepuihyla and Trachycephalus from Brazil, French Guiana, and Peru. Jeff Streicher and Bo Delling provided photographs of the holotype of Hyla quadrangulum and Hyla macrotis, respectively. For the loan of specimens and access to collections we are indebted to D. Frost, D. Kizirian, and R. Pascocello (American Museum of Natural History), R. McDiarmid and K. de Queiroz (National Museum of Natural History), J. C. Señaris and Fernando Rojas (Museo de Historia Natural la Salle), and Barry Clarke (Natural History Museum of London). Antoine Fouquet shared tissues of Trachycephalus from French Guyana. For the collection of specimens we thank S. Aldás, G. Arévalo, F. P. Ayala, J. Ayarzagüena, J. Brito, E. Carrillo, W. E. Duellman, D. Lombeida, J. Manya, A. Mármol, S. Padilla, P. Picerno, J. Pinto, D. Quirola, M. Read, M. Reyes, F. J. M. Rojas, P. Romero, L. Santi, J. C. Señaris, D. Toquetón, A. Varela, and F. F. Velásquez. Ministerio de Ambiente del Ecuador issued collecting permit MAE-DPAP-PIC-FLO-FAU-2015-003. Fieldwork in the headwaters of Ere and Campuya rivers (PJV and GGU) was part of a Rapid Biological Inventory led by the Field Museum, Chicago. PJV is especially indebted to A. Del Campo by logistic support in the field. The advertisement call of Tepuihyla shushupe was recorded with equipment donated by the Cornell Lab of ornithology, Cornell University. Celsi Señaris and F. Rojas-Runjaic took morphological measurements of specimens.
| Funders | Funder number |
|---|---|
| PUCE-DGA | |
| American Museum of Natural History | |
| National Museum of Natural History | |
| Cornell University | |
| Ministerio del Ambiente, Agua y Transición Ecológica | |
| Secretaría de Educación Superior, Ciencia, Tecnología e Innovación |
Keywords
- Advertisement call
- Amazon basin
- Biodiversity
- Ecuador
- Lophiohylini
- Peru
- Phylogeny
- Tepuihyla