Quantitative phase microscopy for the study of bone cells on multiple roughness surfaces

Jesús González-Laprea, Ana H. Márquez, Karem Noris-Suarez, Rafael Escalona

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

Abstract

We present a phase shifting interferometry system for the study of the early adhesion process for osteoblast-like cells, through an interference microscope. Optical phase maps from the cells are obtained experimentally as a function of cell adhesion time. The process is carried out on surfaces of metallic materials relevant to the development of bone implants. The surfaces were subjected to various levels of mechanical polishing and their roughness was measured using the same experimental technique mentioned before. Morphological changes of the cell can be measured over their optical phase maps while the cell adhesion process is accomplished. The experimental technique shows a suitable feature as to the observed time scale, and also shows a high stockiness and precision for the determination of the optical phase.

Original languageEnglish
Title of host publicationInterferometry XVI
Subtitle of host publicationApplications
DOIs
StatePublished - 2012
Externally publishedYes
EventInterferometry XVI: Applications - San Diego, CA, United States
Duration: 13 Aug 201216 Aug 2012

Publication series

NameProceedings of SPIE - The International Society for Optical Engineering
Volume8494
ISSN (Print)0277-786X

Conference

ConferenceInterferometry XVI: Applications
Country/TerritoryUnited States
CitySan Diego, CA
Period13/08/1216/08/12

Keywords

  • Bone cells
  • Interference microscopy
  • Optical phase maps

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