Infection dynamics of batrachochytrium dendrobatidis in two frog species inhabiting quito’s metropolitan guangÜiltagua park, Ecuador

David A. Narváez-Narváez, Alejandro Cabrera-Andrade, Andrés Merino-Viteri, César Paz-Y-miño, Germán Burgos, Alexander Genoy-Puerto

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1 Scopus citations

Abstract

Batrachochytrium dendrobatidis (Bd) infection is one of the principal causes of amphibian declines worldwide. The presence of Bd has been determined in Gastrotheca riobambae tadpoles that inhabit ponds in Quito’s Metropolitan Guangüiltagua Park, Ecuador. This study sought to determine whether these tadpoles are infected and to determine the presence of chytridiomycosis in another frog species, Pristimantis unistrigatus, which also inhabits the park and has different reproductive biology and distinct behavioral habits. We used end-point and real-time PCR techniques to detect and quantify Bd infection. At 1 yr, samples were taken from the skin of P. unistrigatus using swabs and were also taken from the mouthparts of G. riobambae tadpoles. It was found that the two species were infected with a Bd prevalence of 39% (53/135) in G. riobambae tadpoles and 15% (57/382) in P. unistrigatus frogs. The two types of samples (tissue and swabs) from mouthparts showed differences in the zoospores per microliter loads (¯x=1,376.7±3,450.2 vs. ¯x=285.0±652.3). Moreover, a correlation (r2=0.621) was discovered between the monthly mean maximum temperature of the pond with disease prevalence in G. riobambae tadpoles. Infection levels in the P. unistrigatus population varied significantly over time, and distance to the pond was a determinant factor for infection intensity.

Original languageEnglish
Pages (from-to)749-760
Number of pages12
JournalJournal of Wildlife Diseases
Volume57
Issue number4
DOIs
StatePublished - Oct 2021

Bibliographical note

Publisher Copyright:
© Wildlife Disease Association 2021.

Keywords

  • Amphibians declines
  • Chytridiomycosis infection dynamics
  • Gastrotheca riobambae
  • Pristimantis unistrigatus
  • Urban parks

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