Indirect Shoot Regeneration Using 2,4-D Induces Somaclonal Variations in Cinchona Officinalis

Rosa Armijos-González; Luisa Espinosa-Delgado; Augusta Cueva-Agila

doi:10.1590/2179-8087-FLORAM-2021-0017

Indirect Shoot Regeneration Using 2,4-D Induces Somaclonal Variations in Cinchona Officinalis

Rosa Armijos-González, Luisa Espinosa-Delgado, Augusta Cueva-Agila

PUCE Ibarra

Research output: Contribution to journal › Article › peer-review

8 Scopus citations

Abstract

Cinchona officinalis is an important species from the Andean cloud forest that has a low regeneration rate in natural populations. In vitro regeneration of C. officinalis has been successfully established but somaclonal variation was not evaluated. The regeneration pathway and the number of subcultures on somaclonal variation were evaluated using six ISSR primers that amplified 58 loci of Inter Simple Sequence Repeats (ISSR). A dendrogram based on Jaccard´s genetic distance between the subcultures and the donor plant was produced. The results show that indirect shoot regeneration induces somaclonal variation, in the presence 2,4-Dichlorophenoxyacetic acid (2,4-D) in combination with kinetin and 6-Benzylaminopurine (BAP). In combination with 1-Naphthaleneacetic acid (NAA) or with Indole-3-butyric acid (IBA), BAP produces genetically stable explants. The highest proliferation rate was achieved using BAP and IBA. The present research study suggests avoiding the use of 2,4-D when C. officinalis is propagated for reintroduction and restoration projects.

Original language	English
Article number	e20210017
Pages (from-to)	1-8
Number of pages	8
Journal	Floresta e Ambiente
Volume	28
Issue number	3
DOIs	https://doi.org/10.1590/2179-8087-FLORAM-2021-0017
State	Published - 2021

Bibliographical note

Publisher Copyright:
Creative Commons License. All the contents of this journal, except where otherwise noted, is licensed under a Creative Commons Attribution License.

Keywords

auxin
In vitro regeneration
ISSR
subcultures

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1590/2179-8087-FLORAM-2021-0017

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title = "Indirect Shoot Regeneration Using 2,4-D Induces Somaclonal Variations in Cinchona Officinalis",

abstract = "Cinchona officinalis is an important species from the Andean cloud forest that has a low regeneration rate in natural populations. In vitro regeneration of C. officinalis has been successfully established but somaclonal variation was not evaluated. The regeneration pathway and the number of subcultures on somaclonal variation were evaluated using six ISSR primers that amplified 58 loci of Inter Simple Sequence Repeats (ISSR). A dendrogram based on Jaccard´s genetic distance between the subcultures and the donor plant was produced. The results show that indirect shoot regeneration induces somaclonal variation, in the presence 2,4-Dichlorophenoxyacetic acid (2,4-D) in combination with kinetin and 6-Benzylaminopurine (BAP). In combination with 1-Naphthaleneacetic acid (NAA) or with Indole-3-butyric acid (IBA), BAP produces genetically stable explants. The highest proliferation rate was achieved using BAP and IBA. The present research study suggests avoiding the use of 2,4-D when C. officinalis is propagated for reintroduction and restoration projects.",

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AU - Armijos-González, Rosa

AU - Espinosa-Delgado, Luisa

AU - Cueva-Agila, Augusta

N1 - Publisher Copyright: Creative Commons License. All the contents of this journal, except where otherwise noted, is licensed under a Creative Commons Attribution License.

PY - 2021

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N2 - Cinchona officinalis is an important species from the Andean cloud forest that has a low regeneration rate in natural populations. In vitro regeneration of C. officinalis has been successfully established but somaclonal variation was not evaluated. The regeneration pathway and the number of subcultures on somaclonal variation were evaluated using six ISSR primers that amplified 58 loci of Inter Simple Sequence Repeats (ISSR). A dendrogram based on Jaccard´s genetic distance between the subcultures and the donor plant was produced. The results show that indirect shoot regeneration induces somaclonal variation, in the presence 2,4-Dichlorophenoxyacetic acid (2,4-D) in combination with kinetin and 6-Benzylaminopurine (BAP). In combination with 1-Naphthaleneacetic acid (NAA) or with Indole-3-butyric acid (IBA), BAP produces genetically stable explants. The highest proliferation rate was achieved using BAP and IBA. The present research study suggests avoiding the use of 2,4-D when C. officinalis is propagated for reintroduction and restoration projects.

AB - Cinchona officinalis is an important species from the Andean cloud forest that has a low regeneration rate in natural populations. In vitro regeneration of C. officinalis has been successfully established but somaclonal variation was not evaluated. The regeneration pathway and the number of subcultures on somaclonal variation were evaluated using six ISSR primers that amplified 58 loci of Inter Simple Sequence Repeats (ISSR). A dendrogram based on Jaccard´s genetic distance between the subcultures and the donor plant was produced. The results show that indirect shoot regeneration induces somaclonal variation, in the presence 2,4-Dichlorophenoxyacetic acid (2,4-D) in combination with kinetin and 6-Benzylaminopurine (BAP). In combination with 1-Naphthaleneacetic acid (NAA) or with Indole-3-butyric acid (IBA), BAP produces genetically stable explants. The highest proliferation rate was achieved using BAP and IBA. The present research study suggests avoiding the use of 2,4-D when C. officinalis is propagated for reintroduction and restoration projects.

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Indirect Shoot Regeneration Using 2,4-D Induces Somaclonal Variations in Cinchona Officinalis

Abstract

Bibliographical note

Keywords

UN SDGs

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