Genetic diversity and drug resistance of Mycobacterium tuberculosis in Ecuador

J. Zurita; N. Espinel; P. Barba; D. Ortega-Paredes; C. Zurita-Salinas; Y. Rojas; I. Alcocer

doi:10.5588/ijtld.18.0095

Genetic diversity and drug resistance of Mycobacterium tuberculosis in Ecuador

J. Zurita^*, N. Espinel, P. Barba, D. Ortega-Paredes, C. Zurita-Salinas, Y. Rojas, I. Alcocer

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

15 Scopus citations

Abstract

BACKGROUND: The genetic diversity of Mycobacterium tuberculosis in Quito, Ecuador is not well known. OBJECTIVE : To investigate mutations related to drug resistance and bacterial genotypes in M. tuberculosis strains in Ecuador. DESIGN: This was a retrospective study of M. tuberculosis isolates from 104 patients. Isolates were phenotypically resistant to rifampicin (RMP) and/or isoniazid (INH). The genotype was determined using 24-locus mycobacterial interspersed repetitive units-variablenumber tandem repeats (MIRU-VNTR). RESULTS : Isolates showed mutations in the rpoB and katG genes, and the inhA promoter. In rpoB, we found 13 genetic alterations at codons 511, 513, 514, 515, 516, 526 and 531. Forty-six (44.2%) RMP-resistant isolates belonged to codon 531. In katG, there were nine genetic alterations at codons 296, 312, 314, 315, 322, 324 and 351. Fifty-three (51%) INH-resistant isolates belonged to codon 315. Five mutations not previously described were identified in katG: Thr324Ser, Thr314Ala, Ala312Pro, Trp351Stop and deleted G at 296 codon. The Latin American Mediterranean (LAM) (33.7%) and Ghana (30.8%) lineages presented most of the main mutations observed. CONCLUSION: This is the first report from Ecuador; it describes five new mutations in katG and indicates that LAM is the most prevalent lineage.

Original language	English
Pages (from-to)	166-173
Number of pages	8
Journal	International Journal of Tuberculosis and Lung Disease
Volume	23
Issue number	2
DOIs	https://doi.org/10.5588/ijtld.18.0095
State	Published - 1 Feb 2019

Bibliographical note

Publisher Copyright:
© 2019 The Union.

Funding

This study was supported by Pontificia Universidad Católica de Ecuador School of Medicine, Quito (Project L13313) and the Unidad de Investigaciones en Biomedicina of Zurita & Zurita Laboratories, Quito, Ecuador (Project MIC-007). Conflicts of interest: none declared.

Funders	Funder number
Pontificia Universidad Católica de Ecuador School of Medicine, Quito	L13313
Unidad de Investigaciones en Biomedicina of Zurita & Zurita Laboratories, Quito	MIC-007

Keywords

Ecuador
Genotypes
Mycobacterium tuberculosis

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.5588/ijtld.18.0095

Cite this

@article{7ca3496c26a642a085fe0e83a6ae99d6,

title = "Genetic diversity and drug resistance of Mycobacterium tuberculosis in Ecuador",

abstract = "BACKGROUND: The genetic diversity of Mycobacterium tuberculosis in Quito, Ecuador is not well known. OBJECTIVE : To investigate mutations related to drug resistance and bacterial genotypes in M. tuberculosis strains in Ecuador. DESIGN: This was a retrospective study of M. tuberculosis isolates from 104 patients. Isolates were phenotypically resistant to rifampicin (RMP) and/or isoniazid (INH). The genotype was determined using 24-locus mycobacterial interspersed repetitive units-variablenumber tandem repeats (MIRU-VNTR). RESULTS : Isolates showed mutations in the rpoB and katG genes, and the inhA promoter. In rpoB, we found 13 genetic alterations at codons 511, 513, 514, 515, 516, 526 and 531. Forty-six (44.2%) RMP-resistant isolates belonged to codon 531. In katG, there were nine genetic alterations at codons 296, 312, 314, 315, 322, 324 and 351. Fifty-three (51%) INH-resistant isolates belonged to codon 315. Five mutations not previously described were identified in katG: Thr324Ser, Thr314Ala, Ala312Pro, Trp351Stop and deleted G at 296 codon. The Latin American Mediterranean (LAM) (33.7%) and Ghana (30.8%) lineages presented most of the main mutations observed. CONCLUSION: This is the first report from Ecuador; it describes five new mutations in katG and indicates that LAM is the most prevalent lineage.",

keywords = "Ecuador, Genotypes, Mycobacterium tuberculosis",

author = "J. Zurita and N. Espinel and P. Barba and D. Ortega-Paredes and C. Zurita-Salinas and Y. Rojas and I. Alcocer",

note = "Publisher Copyright: {\textcopyright} 2019 The Union.",

year = "2019",

month = feb,

day = "1",

doi = "10.5588/ijtld.18.0095",

language = "English",

volume = "23",

pages = "166--173",

journal = "International Journal of Tuberculosis and Lung Disease",

issn = "1027-3719",

publisher = "International Union Against Tuberculosis and Lung Disease (The Union)",

number = "2",

}

TY - JOUR

T1 - Genetic diversity and drug resistance of Mycobacterium tuberculosis in Ecuador

AU - Zurita, J.

AU - Espinel, N.

AU - Barba, P.

AU - Ortega-Paredes, D.

AU - Zurita-Salinas, C.

AU - Rojas, Y.

AU - Alcocer, I.

PY - 2019/2/1

Y1 - 2019/2/1

N2 - BACKGROUND: The genetic diversity of Mycobacterium tuberculosis in Quito, Ecuador is not well known. OBJECTIVE : To investigate mutations related to drug resistance and bacterial genotypes in M. tuberculosis strains in Ecuador. DESIGN: This was a retrospective study of M. tuberculosis isolates from 104 patients. Isolates were phenotypically resistant to rifampicin (RMP) and/or isoniazid (INH). The genotype was determined using 24-locus mycobacterial interspersed repetitive units-variablenumber tandem repeats (MIRU-VNTR). RESULTS : Isolates showed mutations in the rpoB and katG genes, and the inhA promoter. In rpoB, we found 13 genetic alterations at codons 511, 513, 514, 515, 516, 526 and 531. Forty-six (44.2%) RMP-resistant isolates belonged to codon 531. In katG, there were nine genetic alterations at codons 296, 312, 314, 315, 322, 324 and 351. Fifty-three (51%) INH-resistant isolates belonged to codon 315. Five mutations not previously described were identified in katG: Thr324Ser, Thr314Ala, Ala312Pro, Trp351Stop and deleted G at 296 codon. The Latin American Mediterranean (LAM) (33.7%) and Ghana (30.8%) lineages presented most of the main mutations observed. CONCLUSION: This is the first report from Ecuador; it describes five new mutations in katG and indicates that LAM is the most prevalent lineage.

AB - BACKGROUND: The genetic diversity of Mycobacterium tuberculosis in Quito, Ecuador is not well known. OBJECTIVE : To investigate mutations related to drug resistance and bacterial genotypes in M. tuberculosis strains in Ecuador. DESIGN: This was a retrospective study of M. tuberculosis isolates from 104 patients. Isolates were phenotypically resistant to rifampicin (RMP) and/or isoniazid (INH). The genotype was determined using 24-locus mycobacterial interspersed repetitive units-variablenumber tandem repeats (MIRU-VNTR). RESULTS : Isolates showed mutations in the rpoB and katG genes, and the inhA promoter. In rpoB, we found 13 genetic alterations at codons 511, 513, 514, 515, 516, 526 and 531. Forty-six (44.2%) RMP-resistant isolates belonged to codon 531. In katG, there were nine genetic alterations at codons 296, 312, 314, 315, 322, 324 and 351. Fifty-three (51%) INH-resistant isolates belonged to codon 315. Five mutations not previously described were identified in katG: Thr324Ser, Thr314Ala, Ala312Pro, Trp351Stop and deleted G at 296 codon. The Latin American Mediterranean (LAM) (33.7%) and Ghana (30.8%) lineages presented most of the main mutations observed. CONCLUSION: This is the first report from Ecuador; it describes five new mutations in katG and indicates that LAM is the most prevalent lineage.

KW - Ecuador

KW - Genotypes

KW - Mycobacterium tuberculosis

UR - http://www.scopus.com/inward/record.url?scp=85065412948&partnerID=8YFLogxK

U2 - 10.5588/ijtld.18.0095

DO - 10.5588/ijtld.18.0095

M3 - Article

C2 - 30808448

AN - SCOPUS:85065412948

SN - 1027-3719

VL - 23

SP - 166

EP - 173

JO - International Journal of Tuberculosis and Lung Disease

JF - International Journal of Tuberculosis and Lung Disease

IS - 2

ER -

Genetic diversity and drug resistance of Mycobacterium tuberculosis in Ecuador

Abstract

Bibliographical note

Funding

Keywords

UN SDGs

Access to Document

Other files and links

Fingerprint

Cite this