DOMINANCE OF ST131, B2, BLACTX-M-15, AND PAPA-PAPC-KPSMII-UITA AMONG ESBL ESCHERICHIA COLI ISOLATED FROM BLOODSTREAM INFECTIONS IN QUITO, ECUADOR: A 10-YEAR SURVEILLANCE STUDY (2009–2019): a 10-year surveillance study (2009–2019)

Jeannete Zurita; Gabriela Sevillano; Ariane Paz Y Miño; Nathalí Haro; Marco Larrea-Álvarez; Iliana Alcocer; David Ortega-Paredes

doi:10.1093/jambio/lxad269

DOMINANCE OF ST131, B2, BLACTX-M-15, AND PAPA-PAPC-KPSMII-UITA AMONG ESBL ESCHERICHIA COLI ISOLATED FROM BLOODSTREAM INFECTIONS IN QUITO, ECUADOR: A 10-YEAR SURVEILLANCE STUDY (2009–2019): a 10-year surveillance study (2009–2019)

Jeannete Zurita
, Gabriela Sevillano
, Ariane Paz Y Miño
, Nathalí Haro
, Marco Larrea-Álvarez
, Iliana Alcocer
, David Ortega-Paredes

Research output: Contribution to journal › Article › peer-review

7 Scopus citations

Abstract

Aims: This study aimed to examine antibiotic resistance and the epidemiology of extended-spectrum β-lactamases (ESBL)-producing Escherichia coli associated with bloodstream infections over a period of 10 years. Methods and results: Isolates were collected from January 2009 to December 2019 and those testing for E. coli were included. Antibiotic susceptibility was tested using the VITEK^® system. Selected isolates were further characterized by amplification of marker genes (virulence traits, phylogroups, and sequence types). A total of 166 ESBL-producing E. coli were recovered. The bla_CTX-M-15 allele was the most abundant. Most of the isolates were resistant to ceftriaxone, cefepime, ceftazidime, ampicillin/sulbactam, piperacillin/tazobactam, and ciprofloxacin. No resistance to carbapenems was registered. More than 80% of bacteria were classified as extraintestinal pathogenic E. coli (ExPEC), and the combination of virulence traits: papA-papC-kpsMII-uitA was the most common. Phylogroup B2 was the most prevalent, and bacteria predominantly belonged to ST131. Conclusions: There was an increase in the ExPEC ESBL-E coli in bloodstream infections and the relationship between the isolates found in these infections during these 10 years.

Original language	English
Article number	lxad269
Journal	Journal of Applied Microbiology
Volume	134
Issue number	11
DOIs	https://doi.org/10.1093/jambio/lxad269
State	Published - 16 Nov 2023

Bibliographical note

Publisher Copyright:
© The Author(s) 2023. Published by Oxford University Press on behalf of Applied Microbiology International. All rights reserved.

Funding

The authors are grateful to Microbiology and Tuberculosis Deparment of Vozandes Hospital. A special thanks to Dr Jose Luis Recalde and Dr Camilo Zurita-Salinas. Part of this work was presented in Paris, France at the ECCMID Meeting in April, 2020. This study was supported by the grant QINV0213-IINV533010100 of the Pontificia Universidad Católica del Ecuador and the grant MIC-012 of the Department of Biomedical Research from Zurita & Zurita Laboratories.

Funders	Funder number
Zurita & Zurita Laboratories
Pontifical Catholic University of Ecuador	MIC-012

Keywords

BSI
E. coli
ST131
bla
surveillance

Access to Document

10.1093/jambio/lxad269

Cite this

Zurita, J., Sevillano, G., Paz Y Miño, A., Haro, N., Larrea-Álvarez, M., Alcocer, I., & Ortega-Paredes, D. (2023). DOMINANCE OF ST131, B2, BLACTX-M-15, AND PAPA-PAPC-KPSMII-UITA AMONG ESBL ESCHERICHIA COLI ISOLATED FROM BLOODSTREAM INFECTIONS IN QUITO, ECUADOR: A 10-YEAR SURVEILLANCE STUDY (2009–2019): a 10-year surveillance study (2009–2019). Journal of Applied Microbiology, 134(11), Article lxad269. https://doi.org/10.1093/jambio/lxad269

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title = "DOMINANCE OF ST131, B2, BLACTX-M-15, AND PAPA-PAPC-KPSMII-UITA AMONG ESBL ESCHERICHIA COLI ISOLATED FROM BLOODSTREAM INFECTIONS IN QUITO, ECUADOR: A 10-YEAR SURVEILLANCE STUDY (2009–2019): a 10-year surveillance study (2009–2019)",

abstract = "Aims: This study aimed to examine antibiotic resistance and the epidemiology of extended-spectrum β-lactamases (ESBL)-producing Escherichia coli associated with bloodstream infections over a period of 10 years. Methods and results: Isolates were collected from January 2009 to December 2019 and those testing for E. coli were included. Antibiotic susceptibility was tested using the VITEK{\textregistered} system. Selected isolates were further characterized by amplification of marker genes (virulence traits, phylogroups, and sequence types). A total of 166 ESBL-producing E. coli were recovered. The blaCTX-M-15 allele was the most abundant. Most of the isolates were resistant to ceftriaxone, cefepime, ceftazidime, ampicillin/sulbactam, piperacillin/tazobactam, and ciprofloxacin. No resistance to carbapenems was registered. More than 80\% of bacteria were classified as extraintestinal pathogenic E. coli (ExPEC), and the combination of virulence traits: papA-papC-kpsMII-uitA was the most common. Phylogroup B2 was the most prevalent, and bacteria predominantly belonged to ST131. Conclusions: There was an increase in the ExPEC ESBL-E coli in bloodstream infections and the relationship between the isolates found in these infections during these 10 years.",

keywords = "BSI, E. coli, ST131, bla, surveillance",

author = "Jeannete Zurita and Gabriela Sevillano and \{Paz Y Mi{\~n}o\}, Ariane and Nathal{\'i} Haro and Marco Larrea-{\'A}lvarez and Iliana Alcocer and David Ortega-Paredes",

year = "2023",

month = nov,

day = "16",

doi = "10.1093/jambio/lxad269",

language = "English",

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Zurita, J, Sevillano, G, Paz Y Miño, A, Haro, N, Larrea-Álvarez, M, Alcocer, I & Ortega-Paredes, D 2023, 'DOMINANCE OF ST131, B2, BLACTX-M-15, AND PAPA-PAPC-KPSMII-UITA AMONG ESBL ESCHERICHIA COLI ISOLATED FROM BLOODSTREAM INFECTIONS IN QUITO, ECUADOR: A 10-YEAR SURVEILLANCE STUDY (2009–2019): a 10-year surveillance study (2009–2019)', Journal of Applied Microbiology, vol. 134, no. 11, lxad269. https://doi.org/10.1093/jambio/lxad269

DOMINANCE OF ST131, B2, BLACTX-M-15, AND PAPA-PAPC-KPSMII-UITA AMONG ESBL ESCHERICHIA COLI ISOLATED FROM BLOODSTREAM INFECTIONS IN QUITO, ECUADOR: A 10-YEAR SURVEILLANCE STUDY (2009–2019): a 10-year surveillance study (2009–2019). / Zurita, Jeannete; Sevillano, Gabriela; Paz Y Miño, Ariane et al.
In: Journal of Applied Microbiology, Vol. 134, No. 11, lxad269, 16.11.2023.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - DOMINANCE OF ST131, B2, BLACTX-M-15, AND PAPA-PAPC-KPSMII-UITA AMONG ESBL ESCHERICHIA COLI ISOLATED FROM BLOODSTREAM INFECTIONS IN QUITO, ECUADOR: A 10-YEAR SURVEILLANCE STUDY (2009–2019)

T2 - a 10-year surveillance study (2009–2019)

AU - Zurita, Jeannete

AU - Sevillano, Gabriela

AU - Paz Y Miño, Ariane

AU - Haro, Nathalí

AU - Larrea-Álvarez, Marco

AU - Alcocer, Iliana

AU - Ortega-Paredes, David

PY - 2023/11/16

Y1 - 2023/11/16

N2 - Aims: This study aimed to examine antibiotic resistance and the epidemiology of extended-spectrum β-lactamases (ESBL)-producing Escherichia coli associated with bloodstream infections over a period of 10 years. Methods and results: Isolates were collected from January 2009 to December 2019 and those testing for E. coli were included. Antibiotic susceptibility was tested using the VITEK® system. Selected isolates were further characterized by amplification of marker genes (virulence traits, phylogroups, and sequence types). A total of 166 ESBL-producing E. coli were recovered. The blaCTX-M-15 allele was the most abundant. Most of the isolates were resistant to ceftriaxone, cefepime, ceftazidime, ampicillin/sulbactam, piperacillin/tazobactam, and ciprofloxacin. No resistance to carbapenems was registered. More than 80% of bacteria were classified as extraintestinal pathogenic E. coli (ExPEC), and the combination of virulence traits: papA-papC-kpsMII-uitA was the most common. Phylogroup B2 was the most prevalent, and bacteria predominantly belonged to ST131. Conclusions: There was an increase in the ExPEC ESBL-E coli in bloodstream infections and the relationship between the isolates found in these infections during these 10 years.

AB - Aims: This study aimed to examine antibiotic resistance and the epidemiology of extended-spectrum β-lactamases (ESBL)-producing Escherichia coli associated with bloodstream infections over a period of 10 years. Methods and results: Isolates were collected from January 2009 to December 2019 and those testing for E. coli were included. Antibiotic susceptibility was tested using the VITEK® system. Selected isolates were further characterized by amplification of marker genes (virulence traits, phylogroups, and sequence types). A total of 166 ESBL-producing E. coli were recovered. The blaCTX-M-15 allele was the most abundant. Most of the isolates were resistant to ceftriaxone, cefepime, ceftazidime, ampicillin/sulbactam, piperacillin/tazobactam, and ciprofloxacin. No resistance to carbapenems was registered. More than 80% of bacteria were classified as extraintestinal pathogenic E. coli (ExPEC), and the combination of virulence traits: papA-papC-kpsMII-uitA was the most common. Phylogroup B2 was the most prevalent, and bacteria predominantly belonged to ST131. Conclusions: There was an increase in the ExPEC ESBL-E coli in bloodstream infections and the relationship between the isolates found in these infections during these 10 years.

KW - BSI

KW - E. coli

KW - ST131

KW - bla

KW - surveillance

UR - https://www.scopus.com/pages/publications/85178494745

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DO - 10.1093/jambio/lxad269

M3 - Article

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AN - SCOPUS:85178494745

SN - 1364-5072

VL - 134

JO - Journal of Applied Microbiology

JF - Journal of Applied Microbiology

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M1 - lxad269

ER -

Zurita J, Sevillano G, Paz Y Miño A, Haro N, Larrea-Álvarez M, Alcocer I et al. DOMINANCE OF ST131, B2, BLACTX-M-15, AND PAPA-PAPC-KPSMII-UITA AMONG ESBL ESCHERICHIA COLI ISOLATED FROM BLOODSTREAM INFECTIONS IN QUITO, ECUADOR: A 10-YEAR SURVEILLANCE STUDY (2009–2019): a 10-year surveillance study (2009–2019). Journal of Applied Microbiology. 2023 Nov 16;134(11):lxad269. doi: 10.1093/jambio/lxad269

DOMINANCE OF ST131, B2, BLACTX-M-15, AND PAPA-PAPC-KPSMII-UITA AMONG ESBL ESCHERICHIA COLI ISOLATED FROM BLOODSTREAM INFECTIONS IN QUITO, ECUADOR: A 10-YEAR SURVEILLANCE STUDY (2009–2019): a 10-year surveillance study (2009–2019)

Abstract

Bibliographical note

Funding

Keywords

Access to Document

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Cite this