Development of gastro-resistant coated probiotic granulates and evaluation of viability and release during simulated upper gastrointestinal transit

Natashia Mai Yde Jacobsen; Hanne Bjørn Nedergaard; Anette Kock; Ibrahim Caglayan; Marie Munch Laursen; Eva Marie Lange; Martín Sebastián Marcial-Coba; Daniel Bar-Shalom; Dennis Sandris Nielsen; Anette Müllertz

doi:10.1016/j.lwt.2021.111174

Development of gastro-resistant coated probiotic granulates and evaluation of viability and release during simulated upper gastrointestinal transit

Natashia Mai Yde Jacobsen, Hanne Bjørn Nedergaard, Anette Kock, Ibrahim Caglayan, Marie Munch Laursen, Eva Marie Lange, Martín Sebastián Marcial-Coba, Daniel Bar-Shalom, Dennis Sandris Nielsen, Anette Müllertz^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

4 Scopus citations

Abstract

Probiotics have become one of the most consumed food supplements worldwide. Many probiotic strains are sensitive to low pH and bile concentrations encountered in the gastrointestinal tract upon oral ingestion. This study aimed at developing gastro-resistant probiotic granulates releasing viable cells in the small intestine. Fatty alcohols were investigated as potential coating materials. Cetostearyl alcohol was selected and combined with different plasticizers to further optimize the coating properties. Combining cetostearyl alcohol with olive oil and beeswax in selected concentrations was found promising, and the coatings were applied to L. acidophilus LA3 and B. longum BB536, using hot-melt fluid bed coating. Viability in and release from the coated probiotic granulate was investigated, using a physiological relevant in vitro model simulating conditions in the human stomach, duodenum, jejunum and ileum. L. acidophilus LA3 coated with cetostearyl alcohol and olive oil in the ratio 95:5 (w/w) resulted in significantly higher viability after simulated gastrointestinal transit, compared to the uncoated probiotic powder. Furthermore, the coating showed no release of viable cells after simulated gastric transit. Released viable cells were detected after the remaining steps in the in vitro model, indicating that the coating system provides gastric protection and release during intestinal transit.

Original language	English
Article number	111174
Journal	LWT
Volume	144
DOIs	https://doi.org/10.1016/j.lwt.2021.111174
State	Published - Jun 2021
Externally published	Yes

Bibliographical note

Publisher Copyright:
© 2021

Funding

This work was supported by the Innovation Fund Denmark, Grand solutions project “Stable Probiotic Powder Targeted Children and the Elderly” and the Department of Pharmacy, University of Copenhagen .

Funders	Funder number
Department of Pharmacy, University of Copenhagen
Innovationsfonden

Keywords

Fluid bed
Hot-melt coating
In vitro simulation
Probiotic delivery systems
Probiotics

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10.1016/j.lwt.2021.111174

Cite this

Jacobsen, N. M. Y., Nedergaard, H. B., Kock, A., Caglayan, I., Laursen, M. M., Lange, E. M., Marcial-Coba, M. S., Bar-Shalom, D., Nielsen, D. S., & Müllertz, A. (2021). Development of gastro-resistant coated probiotic granulates and evaluation of viability and release during simulated upper gastrointestinal transit. LWT, 144, Article 111174. https://doi.org/10.1016/j.lwt.2021.111174

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abstract = "Probiotics have become one of the most consumed food supplements worldwide. Many probiotic strains are sensitive to low pH and bile concentrations encountered in the gastrointestinal tract upon oral ingestion. This study aimed at developing gastro-resistant probiotic granulates releasing viable cells in the small intestine. Fatty alcohols were investigated as potential coating materials. Cetostearyl alcohol was selected and combined with different plasticizers to further optimize the coating properties. Combining cetostearyl alcohol with olive oil and beeswax in selected concentrations was found promising, and the coatings were applied to L. acidophilus LA3 and B. longum BB536, using hot-melt fluid bed coating. Viability in and release from the coated probiotic granulate was investigated, using a physiological relevant in vitro model simulating conditions in the human stomach, duodenum, jejunum and ileum. L. acidophilus LA3 coated with cetostearyl alcohol and olive oil in the ratio 95:5 (w/w) resulted in significantly higher viability after simulated gastrointestinal transit, compared to the uncoated probiotic powder. Furthermore, the coating showed no release of viable cells after simulated gastric transit. Released viable cells were detected after the remaining steps in the in vitro model, indicating that the coating system provides gastric protection and release during intestinal transit.",

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Development of gastro-resistant coated probiotic granulates and evaluation of viability and release during simulated upper gastrointestinal transit

Abstract

Bibliographical note

Funding

Keywords

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