Development and characterization of microsatellite markers for two subspecies of Handroanthus chrysanthus

Augusta Yadira Cueva-Agila; Carlos Manchego; Cristian Bastidas; Manuel Curto

doi:10.1590/2175-7860202172088

Development and characterization of microsatellite markers for two subspecies of Handroanthus chrysanthus

Augusta Yadira Cueva-Agila^*, Carlos Manchego, Cristian Bastidas, Manuel Curto

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

Abstract

An understanding of the genetic diversity and structure of plant species is essential in order to comprehend the degree of biodiversity loss and to develop successful restoration programs. Handroanthus is an important genus that presents one of the most valuable timbers of South America. Handroanthus chrysanthus is an important species distributed in Central and South America. Microsatellite markers are not previously developed for this species. Ten microsatellites for Handroanthus chrysanthus developed using high-throughput sequencing are presented here. The usefulness of these microsatellite loci for the genetic analysis of subspecies H. chrysanthus subsp. chrysanthus (distributed in coastal dry forests) and subspecies H. chrysanthus subsp. meridionalis (distributed in premontane moist forests) is analyzed. At least eight polymorphic microsatellites are useful for each subspecies, seven of which can be used in both subspecies.

Original language	English
Article number	e00722020
Journal	Rodriguesia
Volume	72
DOIs	https://doi.org/10.1590/2175-7860202172088
State	Published - 2021
Externally published	Yes

Bibliographical note

Publisher Copyright:
© 2021 Instituto de Pesquisas Jardim Botanico do Rio de Janeiro. All rights reserved.

Funding

This work was supported by the Deutsche Forschungsgemeinschaft (DFG) project entitled “Improvement of forest management key strategies: a contribution to conservation and sustainable land use” (No 227674494) and by Universidad Técnica Particular de Loja (Project No. PY1902). The authors thank I. Arnelas, for her contribution in taxonomic subspecies identification; Anabel Cueva, for her help with the map; and Chris Brinegar, for the critical review of the manuscript. This work was supported by the Deutsche Forschungsgemeinschaft (DFG) project entitled ?Improvement of forest management key strategies: a contribution to conservation and sustainable land use? (No 227674494) and by Universidad T?cnica Particular de Loja (Project No. PY1902).

Funders	Funder number
Universidad T?cnica Particular de Loja
Universidad Técnica Particular de Loja	PY1902
Deutsche Forschungsgemeinschaft	227674494

Keywords

Dry forest
Ecuador
Guayacán
Handroanthus chrysanthus
Microsatellites
Premontane forest
Subspecies

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.1590/2175-7860202172088

Cite this

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title = "Development and characterization of microsatellite markers for two subspecies of Handroanthus chrysanthus",

abstract = "An understanding of the genetic diversity and structure of plant species is essential in order to comprehend the degree of biodiversity loss and to develop successful restoration programs. Handroanthus is an important genus that presents one of the most valuable timbers of South America. Handroanthus chrysanthus is an important species distributed in Central and South America. Microsatellite markers are not previously developed for this species. Ten microsatellites for Handroanthus chrysanthus developed using high-throughput sequencing are presented here. The usefulness of these microsatellite loci for the genetic analysis of subspecies H. chrysanthus subsp. chrysanthus (distributed in coastal dry forests) and subspecies H. chrysanthus subsp. meridionalis (distributed in premontane moist forests) is analyzed. At least eight polymorphic microsatellites are useful for each subspecies, seven of which can be used in both subspecies.",

keywords = "Dry forest, Ecuador, Guayac{\'a}n, Handroanthus chrysanthus, Microsatellites, Premontane forest, Subspecies",

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doi = "10.1590/2175-7860202172088",

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volume = "72",

journal = "Rodriguesia",

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AU - Cueva-Agila, Augusta Yadira

AU - Manchego, Carlos

AU - Bastidas, Cristian

AU - Curto, Manuel

PY - 2021

Y1 - 2021

N2 - An understanding of the genetic diversity and structure of plant species is essential in order to comprehend the degree of biodiversity loss and to develop successful restoration programs. Handroanthus is an important genus that presents one of the most valuable timbers of South America. Handroanthus chrysanthus is an important species distributed in Central and South America. Microsatellite markers are not previously developed for this species. Ten microsatellites for Handroanthus chrysanthus developed using high-throughput sequencing are presented here. The usefulness of these microsatellite loci for the genetic analysis of subspecies H. chrysanthus subsp. chrysanthus (distributed in coastal dry forests) and subspecies H. chrysanthus subsp. meridionalis (distributed in premontane moist forests) is analyzed. At least eight polymorphic microsatellites are useful for each subspecies, seven of which can be used in both subspecies.

AB - An understanding of the genetic diversity and structure of plant species is essential in order to comprehend the degree of biodiversity loss and to develop successful restoration programs. Handroanthus is an important genus that presents one of the most valuable timbers of South America. Handroanthus chrysanthus is an important species distributed in Central and South America. Microsatellite markers are not previously developed for this species. Ten microsatellites for Handroanthus chrysanthus developed using high-throughput sequencing are presented here. The usefulness of these microsatellite loci for the genetic analysis of subspecies H. chrysanthus subsp. chrysanthus (distributed in coastal dry forests) and subspecies H. chrysanthus subsp. meridionalis (distributed in premontane moist forests) is analyzed. At least eight polymorphic microsatellites are useful for each subspecies, seven of which can be used in both subspecies.

KW - Dry forest

KW - Ecuador

KW - Guayacán

KW - Handroanthus chrysanthus

KW - Microsatellites

KW - Premontane forest

KW - Subspecies

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DO - 10.1590/2175-7860202172088

M3 - Article

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ER -

Development and characterization of microsatellite markers for two subspecies of Handroanthus chrysanthus

Abstract

Bibliographical note

Funding

Keywords

UN SDGs

Access to Document

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Cite this