TY - JOUR
T1 - COVID-19 outbreak in Malaysia
T2 - Decoding D614G mutation of SARS-CoV-2 virus isolated from an asymptomatic case in Pahang
AU - Mat Yassim, Aini Syahida
AU - Asras, Mohd Fazli Farida
AU - Gazali, Ahmad Mahfuz
AU - Marcial-Coba, Martin S.
AU - Zainulabid, Ummu Afeera
AU - Ahmad, Hajar Fauzan
N1 - Publisher Copyright:
© 2021 Elsevier Ltd. All rights reserved.
PY - 2021
Y1 - 2021
N2 - SARS-CoV-2 is a very transmissible and pathogenic coronavirus which detected in Malaysia in January 2020. Nevertheless, the sample from Malaysia is still under-sequenced. Hence lacking clarity of the circulating strain in Malaysia leads to a deadlock in understanding the virus infectivity. This study aimed to investigate the genome identity of circulating COVID-19 strains in Pahang and understand disease epidemiology during the pandemic. This study leveraged high-throughput sequencing analysis for the whole genome sequencing and implemented bioinformatic technique for the analysis. Here we reported that the virus with D614G mutation in Spike protein circulates in a few Malaysia states before the Sivagangga cluster announced in Kedah in July 2020. This mutated virus includes our virus sample isolated in April 2020 from an asymptomatic patient in Pahang. Based on the phylogenetic analysis, we discovered the origin of our sample Pahang/IIUM91 was not related to Sivagangga cluster. Here, we have generated 3D structure model of Pahang/IIUM91 Spike protein. D614G mutation in Pahang/IIUM91 Spike protein increases viral stability and flexibility, hence render higher infectivity. Collectively, our results suggest for the establishment of a complete SARS-CoV-2 genome database in Malaysia. Hence, more research should be established to learn the behaviour of this virus.
AB - SARS-CoV-2 is a very transmissible and pathogenic coronavirus which detected in Malaysia in January 2020. Nevertheless, the sample from Malaysia is still under-sequenced. Hence lacking clarity of the circulating strain in Malaysia leads to a deadlock in understanding the virus infectivity. This study aimed to investigate the genome identity of circulating COVID-19 strains in Pahang and understand disease epidemiology during the pandemic. This study leveraged high-throughput sequencing analysis for the whole genome sequencing and implemented bioinformatic technique for the analysis. Here we reported that the virus with D614G mutation in Spike protein circulates in a few Malaysia states before the Sivagangga cluster announced in Kedah in July 2020. This mutated virus includes our virus sample isolated in April 2020 from an asymptomatic patient in Pahang. Based on the phylogenetic analysis, we discovered the origin of our sample Pahang/IIUM91 was not related to Sivagangga cluster. Here, we have generated 3D structure model of Pahang/IIUM91 Spike protein. D614G mutation in Pahang/IIUM91 Spike protein increases viral stability and flexibility, hence render higher infectivity. Collectively, our results suggest for the establishment of a complete SARS-CoV-2 genome database in Malaysia. Hence, more research should be established to learn the behaviour of this virus.
KW - D614G
KW - Malaysia
KW - Mutation
KW - Pahang
KW - SARS-CoV-2
KW - Spike protein
UR - http://www.scopus.com/inward/record.url?scp=85112291338&partnerID=8YFLogxK
U2 - 10.1016/j.matpr.2021.02.387
DO - 10.1016/j.matpr.2021.02.387
M3 - Artículo de la conferencia
AN - SCOPUS:85112291338
SN - 2214-7853
VL - 48
SP - 828
EP - 836
JO - Materials Today: Proceedings
JF - Materials Today: Proceedings
ER -