TY - JOUR
T1 - Candida theae sp. nov., a new anamorphic beverage-associated member of the Lodderomyces clade
AU - Chang, Chin Feng
AU - Lin, Yu Ching
AU - Chen, Shan Fu
AU - Carvaja Barriga, Enrique Javier
AU - Barahona, Patricia Portero
AU - James, Stephen A.
AU - Bond, Christopher J.
AU - Roberts, Ian N.
AU - Lee, Ching Fu
PY - 2012/2/1
Y1 - 2012/2/1
N2 - Four strains representing a novel yeast species belonging to the genus Candida were independently isolated in Taiwan and Ecuador. Two strains (G17 T and G31) were isolated in Taiwan, by pellet precipitation from plastic-bottled tea drinks produced in Indonesia, while two additional strains (CLQCA 10-049 and CLQCA 10-062) were recovered from ancient chicha fermentation vessels found in tombs in Quito, Ecuador. These four strains were morphologically, and phylogenetically identical to each other. No sexual reproduction was observed on common sporulation media. Large-subunit (LSU) rRNA gene sequence analysis revealed the four strains to belong to the Lodderomyces clade, closely related to members of the Candida parapsilosis species complex. The four strains, which have identical LSU D1/D2 sequences, differ from their closest phylogenetic neighbors, Candida orthopsilosis and Candida parapsilosis, by 6-9 nt substitutions, respectively. Physiologically, the four strains are similar to Candida parapsilosis, although they can be distinguished from their closest relative by the assimilation of arbutin, nitrite, and creatine. The Indonesian and Ecuadorian strain sets can also be distinguished from one another based on ITS sequencing, differing by 4 substitutions in ITS1 and 1 single nucleotide indel in ITS2. Collectively, the results indicate that the four strains represent a previously unrecognized species of Candida. The name Candida theae sp. nov. is proposed to accommodate these strains, with G-17 T (BCRC 23242 T=CBS 12239 T=ATCC MYA-4746 T) designated as the type strain.
AB - Four strains representing a novel yeast species belonging to the genus Candida were independently isolated in Taiwan and Ecuador. Two strains (G17 T and G31) were isolated in Taiwan, by pellet precipitation from plastic-bottled tea drinks produced in Indonesia, while two additional strains (CLQCA 10-049 and CLQCA 10-062) were recovered from ancient chicha fermentation vessels found in tombs in Quito, Ecuador. These four strains were morphologically, and phylogenetically identical to each other. No sexual reproduction was observed on common sporulation media. Large-subunit (LSU) rRNA gene sequence analysis revealed the four strains to belong to the Lodderomyces clade, closely related to members of the Candida parapsilosis species complex. The four strains, which have identical LSU D1/D2 sequences, differ from their closest phylogenetic neighbors, Candida orthopsilosis and Candida parapsilosis, by 6-9 nt substitutions, respectively. Physiologically, the four strains are similar to Candida parapsilosis, although they can be distinguished from their closest relative by the assimilation of arbutin, nitrite, and creatine. The Indonesian and Ecuadorian strain sets can also be distinguished from one another based on ITS sequencing, differing by 4 substitutions in ITS1 and 1 single nucleotide indel in ITS2. Collectively, the results indicate that the four strains represent a previously unrecognized species of Candida. The name Candida theae sp. nov. is proposed to accommodate these strains, with G-17 T (BCRC 23242 T=CBS 12239 T=ATCC MYA-4746 T) designated as the type strain.
KW - Bottled tea
KW - Chicha
KW - Food spoilage
KW - New species
UR - http://www.scopus.com/inward/record.url?scp=84855467453&partnerID=8YFLogxK
U2 - 10.1016/j.ijfoodmicro.2011.09.012
DO - 10.1016/j.ijfoodmicro.2011.09.012
M3 - Article
C2 - 22088606
AN - SCOPUS:84855467453
SN - 0168-1605
VL - 153
SP - 10
EP - 14
JO - International Journal of Food Microbiology
JF - International Journal of Food Microbiology
IS - 1-2
ER -