A new morphological cell parameter based on optical phase for the evaluation of cell populations

Jesús González-Lapea, Ana H. Márquez, José R. Darias, Karem Noris-Suárez

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

Abstract

In this work, we introduce an opto-geometric parameter for the morphologic characterization of cell populations during their early adhesion process. Using measurements for a cell population of the maximal optical phase for each cell and its substrate-contact-surface, we show, experimentally, that a relationship exists between these variables. This connection is primarily associated with morphological cell characteristics. It is also shown that using the relationship obtained above, we can derive a morphological parameter, which, for the cell populations studied, results in a monodisperse Gaussiansize distribution, which would allow for the use of regular statistical variables. This result is in total contrast with the polydisperse distribution obtained if only the contact surface area between cell and substrate is used. In addition, optical phase measurements where accomplished by phase shifted interferometry using a Mirau-type interference microscope. The cellular system studied consisted of Osteoblast-like cells, plated on 316VM medical-grade stainless steel polished surfaces. These cell populations were studied within the same culture conditions of cell type, plating time and substrate roughness conditions. The existence of a relationship between maximal optical phase and substrate contact area agrees entirely with the accepted spreading model for cell adhesion; in particular, considering the close link between the optical phase time change and cell thickness reduction.

Original languageEnglish
Title of host publicationInterferometry XIX
EditorsKatherine Creath, Jan Burke, Angela D. Davies, Michael B. North Morris, Katherine Creath
PublisherSPIE
ISBN (Electronic)9781510620698
DOIs
StatePublished - 2018
EventInterferometry XIX 2018 - San Diego, United States
Duration: 21 Aug 201823 Aug 2018

Publication series

NameProceedings of SPIE - The International Society for Optical Engineering
Volume10749
ISSN (Print)0277-786X
ISSN (Electronic)1996-756X

Conference

ConferenceInterferometry XIX 2018
Country/TerritoryUnited States
CitySan Diego
Period21/08/1823/08/18

Bibliographical note

Publisher Copyright:
© 2018 SPIE.

Keywords

  • Cell adhesion
  • Cell morphology
  • Interference Microscopy
  • Optical phase
  • Quantitative phase microscopy.

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